Human BMP-2 Recombinant Protein 4697S from Cell Signaling Technology

Product Specs
ItemHuman BMP-2 Recombinant Protein
CompanyCell Signaling Technology
Price Pricing Info Supplier Page View Company Product Page
Catalog Number4697S
Quantity10 µg
Molecule NameBMP2
NCBI Gene AliasesBDA2, BMP2A, SSFSC, SSFSC1
NCBI Full Gene Namebone morphogenetic protein 2
Research AreaDevelopmental Biology
Molecular Weight26
Source/Expression SystemE. Coli

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Cell Signaling Technology

3 Trask Lane

Danvers, Massachusetts 01923

United States

Phone: 877-616-CELL
Phone: 978-867-2388
Fax: 866-432-6112
Fax: 978-867-2488

Company Profile

Website: http://www.cellsignal.com/

Website: Technical Support

(1) Application of Condition Monitoring for Hydraulic Oil Using Tuning Fork Sensor: A Study Case on Hydraulic System of Earth Moving MachineryMaterials (Basel)October 31, 2022Hong-Gyu Jeon, Jong-Kyun Kim, Seon-Jun Na, Min-Seok Kim, Sung-Ho HongSampling(May 2022),3rd Oil Sampling(August 2022) Monitoring A,4495 h,4967 h,5591 h,5848 h Monitoring B,4697 h,5191 h,5808 h,6380 h Monitoring C,5450 h,6040 h,6700 h,7163 h Monitoring D,2109 h,2974 h,3858 h,4641

(2) Betuletol, a Propolis Component, Suppresses IL-33 Gene Expression and Effective against EosinophiliaMoleculesAugust 25, 2022Aurpita Shaha, Rezwanul Islam, Naonobu Tanaka, Yoshiki Kashiwada, Hiroyuki Fukui, Noriaki Takeda, Yoshiaki Kitamura, Hiroyuki Mizuguchi(E-4, sc-7383) were from Santa Cruz Biotechnology (Santa Cruz, CA, USA). The antibody for β-actin (#4697) was from Cell Signaling Technology Japan (Tokyo, Japan). Goat anti-rabbit IgG (H+L)-HRP conjugate (#170-6515)

(3) Senescence Induced by BMI1 Inhibition is a Therapeutic Vulnerability in H3K27M-Mutant DIPGSSRN Electronic JournalApril 2, 2022Ilango Balakrishnan et al.abundance of GFAPpositive cells that is mediated by PTGDS in mouse brain. Hum. Mol. Genet. 24, 4686–4697. Cell Reports 33, 108286, October 20, 2020 19 ll OPEN ACCESS Article STAR+METHODS KEY RESOURCES

(4) Doublecortin in the Fish Visual System, a Specific Protein of Maturing NeuronsBiology (Basel)February 6, 2022Laura DeOliveira-Mello, Isabel Vicente, Veronica Gonzalez-Nunez, Adrian Santos-Ledo, Almudena Velasco, Rosario Arévalo, Juan M. Lara, Andreas F. MackSDS, and TBS-T. β-actin antibody (1:1.000 diluted in blocking buffer; Cell Signalling, Danvers, USA, 4697. Lot nº. 10/2016) was used as loading control. Protein expression was determined by densitometric analysis

(5) Osteoblastic glucocorticoid signaling exacerbates high-fat-diet- induced bone loss and obesityBone ResearchDecember 1, 2021Sarah Kim et al.against sclerostin (0.2 μg·mL−1, #AF1589, R&D Systems, Minneapolis, MN, U.S.A.) or beta-actin (1:1 000, #4697, Cell Signaling Technology, Danvers, MA, U.S.A.). After incubation with appropriate secondary antibodies,

(6) SphK1 functions downstream of IGF-1 to modulate IGF-1-induced EMT, migration and paclitaxel resistance of A549 cells: A preliminary in vitro studyJournal of CancerJuly 10, 2019Xingping Wu, Qibiao Wu, Xiqiao Zhou, Jianan Huang92547), anti-p-ERK1/2 (Cell Signaling Technology Cat# 4377), anti-ERK (Cell Signaling Technology Cat# 4697), anti-p-AKT(S473) (Cell Signaling Technology Cat# 9271 ), anti-AKT (Abcam Cat# AB8805), anti-GAPDH

(7) GluR2 endocytosis-dependent protein degradation in the amygdala mediates memory updatingScientific reportsMarch 26, 2019Ferrara NC, Jarome TJ, Cullen PK, Orsi SA, Kwapis JL, Trask S, Pullins SE, Helmstetter FJ.CA). Antibodies Western blot antibodies included GluR2 (1:1000, NeuroMab #75-002) and Actin (Cell Signaling, 1:1000 #4697) as a loading control. Immunofluorescence antibodies included zif268 (Cell Signaling, 1:500) and Anti-Ubiquitin

(8) ARHGEF4 predicts poor prognosis and promotes cell invasion by influencing ERK1/2 and GSK-3α/β signaling in pancreatic cancerInternational journal of oncologyNovember 1, 2018Taniuchi K, Furihata M, Naganuma S, Saibara T.anti-GAPDH (sc32233) antibodies were purchased from Santa Cruz Biotechnology, Inc. (Dallas, TX, USA). Anti-ERK1/2 (4697) and anti-phosphorylated ERK1/2 (Thr204/Tyr187; 5726) antibodies were purchased from Cell Signaling Technology

(9) Effect of wild grape on the signaling of histamine H₁ receptor gene expression responsible for the pathogenesis of allergic rhinitisThe Journal of Medical InvestigationOctober 5, 2018Rezwanul Islam, Hiroyuki Mizuguchi, Aurpita Shaha, Kohei Nishida, Masami Yabumoto, Hisashi Ikeda, Hiromichi Fujino, Yoshiaki Kitamura, Hiroyuki Fukui, Noriaki Takeda(sc-937, Santa Cruz Biotechnology, Santa Cruz, CA, USA) and phospho-PKCδ (Tyr311) and β-actin (#2055S and #4697, Cell Signaling Technology Japan, Tokyo)] overnight at 4" !. Proteins were visualized with an Immobilon Western Chemiluminescent HRP substrate

(10) Osteocalcin expressing cells from tendon sheaths in mice contribute to tendon repair by activating Hedgehog signalingeLifeDecember 15, 2017Wang Y, Zhang X, Huang H, Xia Y, Yao Y, Mak AF, Yung PS, Chan KM, Wang L, Zhang C, et al.kit,Dual-luciferase reporter assay system,"Promega, USA",E1980 "Chemical compound, drug",BMP2,"CST, USA",#4697 "Chemical compound, drug",TGF-β1,"Peprotech, USA",100–21 "Chemical compound, drug",Purmorphamine,"Millipore,

(11) The target landscape of clinical kinase drugsScience (New York, N.Y.)December 1, 2017Klaeger S, Heinzlmeir S, Wilhelm M, Polzer H, Vick B, Koenig PA, Reinecke M, Ruprecht B, Petzoldt S, Meng C, et al.Gilteritinib 1 µM, Saracatinib 1µM, 100 nM, 10 nM in DMSO) for 30 min prior to addition of 100 ng/ml BMP2 (#4697, Cell signaling technology), followed by 45 min incubation before cells were lysed using an NP40-based

(12) Comparison of two different analysis approaches for DTI free-water corrected and uncorrected maps in the study of white matter microstructural integrity in individuals with depressionHuman Brain MappingSeptember 1, 2017Maurizio Bergamino, Rayus Kuplicki, Teresa A. Victor, Yoon-Hee Cha, Martin P. PaulusL. Fewer clusters were found when RD-FW maps were used. Additionally, with RD maps we found higher 4697 r r Bergamino et al. r TABLE III. Pearson’s correlation coefficients between PHQ-9 scores and

(13) Antipsychotic-induced Hdac2 transcription via NF-κB leads to synaptic and cognitive side effectsNature neuroscienceSeptember 1, 2017Ibi D, de la Fuente Revenga M, Kezunovic N, Muguruza C, Saunders JM, Gaitonde SA, Moreno JL, Ijaz MK, Santosh V, Kozlenkov A, et al.Cruz sc-8030, 1:250), anti-β-actin (mouse brain: Abcam ab8227, 1:3,000 or 1:10,000, Cell Signaling 4697S, 1:5,000; human brain: Sigma-Aldrich, 1:200,000; Neuro-2a cells: Abcam ab8227, 1:3,000, Cell Signaling

(14) SMOC can act as both an antagonist and an expander of BMP signalingeLifeMarch 21, 2017J Terrig Thomas, D Eric Dollins, Kristin R Andrykovich, Tehyen Chu, Brian G Stultz, Deborah A Hursh, Malcolm Moosused at molar equivalent amounts based on their predicted molecular weights. BMP2 (Cell Signaling #4697) was added at 50 ng/ml. Xenopus embryo manipulations Frogs (Xenopus laevis), purchased from NASCO

(15) Pericytes of Multiple Organs Do Not Behave as Mesenchymal Stem Cells In VivoCell stem cellMarch 2, 2017Guimarães-Camboa N, Cattaneo P, Sun Y, Moore-Morris T, Gu Y, Dalton ND, Rockenstein E, Masliah E, Peterson KL, Stallcup WB, et al.(Sigma-Aldrich, 49752), 10 mM β -glycerophosphate (Sigma-Aldrich, G9422), 300 ng/mL BMP2 (Cell Signaling, 4697) and 1% P/S. After 21 days of treatment with differentiation medium, cultures were fixed in 4% PFA for

(16) IGF-IR cooperates with ERα to inhibit breast cancer cell aggressiveness by regulating the expression and localisation of ECM moleculesScientific reportsJanuary 12, 2017Afratis NA, Bouris P, Skandalis SS, Multhaupt HA, Couchman JR, Theocharis AD, Karamanos NK.

(17) Examining Crosstalk among Transforming Growth Factor β, Bone Morphogenetic Protein, and Wnt Pathways*Journal of Biological ChemistryJanuary 6, 2017Adam D. Coster, Curtis A. Thorne, Lani F. Wu, Steven J. Altschuler75%); TGFβ3 (Cell Signaling Technology 8425, 22 kDa (dimer), 98%); BMP4 (Cell Signaling Technology 4697, 26 kDa (dimer), 95%); Dickkopf-1 (R&D Systems 5439-DK, 26 kDa, 95%); Noggin (R&D Systems 6057-NG, 23

(18) Modulation Effect of Peroxisome Proliferator-Activated Receptor Agonists on Lipid Droplet Proteins in LiverJournal of Diabetes ResearchJanuary 1, 2016Yun-Xia Zhu, Ming-Liang Zhang, Yuan Zhong, Chen Wang, Wei-Ping JiaRockford, IL, USA). Protein expression values were standardized against 𝛽-actin protein expression (4697s, Cell Signaling Technology, Danvers, MA, USA). The average intensity for each band was quantified with

(19) Compensatory Islet Response to Insulin Resistance Revealed by Quantitative ProteomicsJournal of proteome researchAugust 7, 2015El Ouaamari A, Zhou JY, Liew CW, Shirakawa J, Dirice E, Gedeon N, Kahraman S, De Jesus DF, Bhatt S, Kim JS, et al.(Cell Signaling, #4850), MAOB (Abcam, ab125010), SDHB (Life technologies, A21345), or β-actin (Cell Signaling, #4697). Densitometry was performed using Image J software. RESULTS Author Manuscript To examine islet proteome

(20) CpG island hypermethylation-associated silencing of microRNAs promotes human endometrial cancerCancer Cell InternationalDecember 1, 2013Bi-Lan Li, Wen Lu, Cong Lu, Jun-jie Qu, Ting-ting Yang, Qin Yan, Xiao-ping Wan(1:1000 dilution, Epitomics), N-cadherin (1:1000 dilution, CST, USA) and β-actin (1:1000 dilution, 4697s, CST, USA). The membranes were washed three times with PBS and then incubated with peroxidase-linked

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Human BMP-2

Human BMP-2 Recombinant Protein from Cell Signaling Technology

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Human BMP-2 Recombinant Protein from Cell Signaling Technology

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